By Ashok Agarwal, Sajal Gupta, Rakesh Sharma
This state of the art laboratory guide comprises 20 medical protocols used day-by-day for the research of the infertile male, awarded with effortless to appreciate, step by step method. The protocols are prepared from regimen to complicated laboratory strategies universal to scientific perform, together with computer-assisted semen research, sperm training for IUI by means of density gradient and swim-up, sperm cryopreservation, and sperm DNA fragmentation try out via TUNEL technique, between others. The method in every one protocol follows top perform instructions made clearer through professionally hand-drawn illustrations masking lots of the vital steps and gear. The authors, hailing from the world-renowned Andrology middle at Cleveland health center, have over 50 years of mixed first-hand event in dealing with very busy diagnostic and study amenities in male infertility and andrology. The publication can be an quintessential source for millions of laboratory technologists, clinicians and reproductive pros (andrologists, embryologist, etc.) engaged within the prognosis and administration of infertile males round the world.
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Extra info for Andrological Evaluation of Male Infertility: A Laboratory Guide
1 (continued) Laboratory procedure Maintenance and QC activities Supply inventory and ordering Receipt of shipments, logging, labeling, and storage of supplies Contact materials log and toxicity testing Testing for microbial contamination Centrifuge maintenance and QC Dewar maintenance and QC CO2 incubator maintenance and QC Biosafety hood maintenance and QC Microscope maintenance and QC Pipettor maintenance and QC Refrigerators and freezer maintenance and QC Thermometer maintenance and QC Weekly, monthly, biannual, annual QC Review of SOPs Storage of laboratory records The event record Communications and complaints Detection and reporting of incidents—incident response Date approved Supervisor initials Comments CLIA Clinical Laboratory Improvement Amendment, AATB American Association of Tissue Banks, HIPPA Health Insurance Portability and Accountability Act, PPE personal protective equipment, ASA antisperm antibody would do in hypothetical situations, what regulations govern his or her current activity, and the performance speciﬁcations for the test being conducted.
A study of semen parameters with emphasis on sperm morphology in a fertile population: an attempt to develop clinical thresholds. Hum Reprod. 2000;16:110–4. 31. Menkveld R, Wong WY, Lombard CJ, Wezels AMM, Thomas CMG, Merkus HMWM, Steegers-Theunissen PM. Semen parameters, including WHO and strict criteria morphology, in a fertile and subfertile population: an effort towards standardization of in-vivo thresholds. Hum Reprod. 2001;16:1165–71. 32. Sergerie M, Laforest G, Bujan L, Bissonnette F, Bleau G.
Precision is a measure of the ability of a test to give the same result repeatedly for the same sample. 11. The standard deviations and coefﬁcients of variations of replicate counts of individual patient specimens are measures of the precision of sperm count determination. MicroCell chambers are more precise than hemocytometers , while Makler chambers require very strict QC to achieve accurate and precise results. If loaded correctly, with the cover glass seated ﬁrmly on the silicone posts, the Makler chamber produces slightly lower concentration values than hemocytometry, although the precision is comparable if daily QC is performed.
Andrological Evaluation of Male Infertility: A Laboratory Guide by Ashok Agarwal, Sajal Gupta, Rakesh Sharma